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. 2012 Oct 2;7(10):e46623. doi: 10.1371/journal.pone.0046623

Table 4. ORFs in the Unique Long sequence and their predicted features.

Designation Map position Predicted features
F-UL0.5 c(32683..33594) Putative nuclear protein
F-UL01 34789..35169 Glycoprotein L (gL)
F-UL02 35138..35980 Uracil DNA glycosylase (UDG)
F-UL03 36025..36708 Nuclear phosphoprotein
F-UL04 c(37192..37779) Nuclear protein
F-UL05 c(37823..40357) Putative component of DNA helicase/primase complex; helicase signature
HP14 38921..39544 Similarity to hypothetical protein of Gallid herpesvirus 1
F-UL06 40356..42338 Putative capsid portal protein
HP15 41039..41554 Hypothetical nuclear protein
F-UL07 42211..43125 Herpes_UL7 superfamily
F-UL08 c(43118..45361) Putative UL8 Herpesvirus DNA helicase/primase complex associated protein
F-UL09 c(45306..47780) Putative Origin-binding protein; Domain: DEAD-like helicases superfamily; ATP binding site on conserved domain DEXDc; putative Mg++ binding site on conserved domain DEXDc.
F-UL10 47779..49044 Glycoprotein M (gM); 99% identical to AAU84515.1 in GenBank
F-UL11 c(49134..49418) Putative myristylated protein
F-UL12 c(49325..51004) 99% identity with gb|AAU84516.1| UL12 [Fibropapilloma-associated turtle herpesvirus]; YqaJ-like viral recombinase domain; herpesvirus alkaline exonuclease
HP16 50429..51067 Hypothetical protein; predicted bipartite NLS
F-UL14 c(51058..51594) 96% identity with gb|AAU84517.1| UL14 [Fibropapilloma-associated turtle herpesvirus]
F-UL15A 51593..52606 Probable DNA packing protein, N-terminus; 97% identity with gb|AAU84518.1| UL15A [Fibropapilloma-associated turtle herpesvirus]
F-UL16 c(52618..53673) 97% identical to gb|AAU84520.1| UL16 [Fibropapilloma-associated turtle herpesvirus]; herpesvirus UL16/UL94 family; capsid maturation, DNA packaging/cleavage.
F-UL17 c(53613..55568) DNA packaging tegument protein; Multi domain PFAM: pfam04559, herpesvirus UL17 protein; 96% identity to gb|AAU84521.1| UL17 [Fibropapilloma-associated turtle herpesvirus]
F-UL15B 55718..56788 Probable DNA packing protein, C-terminus; 99% identity to gb|AAU84519.1| UL15B [Fibropapilloma-associated turtle herpesvirus]
HP17 57297..57764 Hypothetical protein
HP18 58434..59129 Unknown Protein; Similarity to protein RL5A [Human herpesvirus 5] gb|AAO48775.1|
HP19 58933..59415 Hypothetical protein; E value: 0.035: gb|AAR31234.1| protein RL6 [Human herpesvirus 5]
HP20 59950..60450 Hypothetical protein; E value 0.017: U54 HHV6B; ref|NP_050235.1| Gene info linked to NP_050235.1 virion transactivator [Human herpesvirus 6]
F-UL18 c(60772..61764) 99% identity to UL18 [Fibropapilloma-associated turtle herpesvirus] gb|AAU84522.1|; VP23 “capsid triplex subunit 2”
F-UL19 c(61835..65986) Major capsid protein; 99% identity to gb|AAU84523.1| major capsid protein [Fibropapilloma-associated turtle herpesvirus]
F-UL20 c(66072..66701) 100% identical with gb|AAU84524.1| UL20 [Fibropapilloma-associated turtle herpesvirus]; Herpesvirus egress protein UL20; 5 TM helices predicted
F-UL21 66784..68187 85% identity with gb|AAU84525.1| UL21 tegument protein [Fibropapilloma-associated turtle herpesvirus]
F-UL22 c(68248..70485) Putative glycoprotein H (gH); 98% identity to gb|AAU84526.1| glycoprotein H [Fibropapilloma-associated turtle herpesvirus]; predicted features: signal sequence; 4 N-gly sites; transmembrane helix
F-UL23 c(70555..71637) 98% identity to gb|AAU84527.1| thymidine kinase [Fibropapilloma-associated turtle herpesvirus]; C-terminal extension relative to gb|AAU93321.1| thymidine kinase [Hawaiian green turtle herpesvirus]
F-UL24 71586..72527 PFAM: cl03293 [Superfamily] cl03293, Herpes virus protein UL24; 100% identity with gb|AAU93322.1| membrane-associated protein [Hawaiian green turtle herpesvirus]; 98% identity with gb|AAU84528.1| UL24 [Fibropapilloma-associated turtle herpesvirus]
F-UL25 72184..73857 99% identity with gb|AAU93323.1| minor capsid protein [Hawaiian green turtle herpesvirus]; 98% identity with gb|AAU84529.1| UL25 [Fibropapilloma-associated turtle herpesvirus]
HP21 c(72282..72755) Hypothetical protein; predicted NLS
F-UL26 73935..75584 Putative UL26 capsid maturation protease"; 100% identity with gb|AAU93324.1| capsid maturation protease [Hawaiian green turtle herpesvirus]; 99% identity with gb|AAU84530.1| UL26 [Fibropapilloma-associated turtle herpesvirus]
F-UL26.5 74703..75584 Putative UL26.5 virion scaffolding protein; 100% identity with gb|AAU93325.1| virion scaffolding protein [Hawaiian green turtle herpesvirus]
F-UL27 c(75736..78291) Glycoprotein B (gB); 100% identity with gb|AAU93326.1| virion membrane glycoprotein B [Hawaiian green turtle herpesvirus]; 99% identity with gb|AAU84531.1| glycoprotein B [Fibropapilloma-associated turtle herpesvirus]; predicted features: signal peptide; 3 transmembrane helices
HP22 76522..77211 Hypothetical protein
HP23 77350..78219 Limited similarity to C3 Complement C3 precursor protein
F-UL28 c(78288..80540) ICP18.5; 99% identity with gb|AAU93327.1| DNA cleavage/packaging protein [Hawaiian green turtle herpesvirus]
F-UL29 c(80728..84312) Putative UL29 single-stranded DNA binding protein [Green turtle herpesvirus]; [Superfamily] cl09516, ssDNA binding protein; 99% identity with gb|AAQ67362.1| single-stranded DNA binding protein [Green turtle herpesvirus]
HP24 83548..84489 6-phosphofructokinase-like hypothetical protein; NLS predicted
F-UL30 84473..87925 Putative DNA polymerase catalytic subunit [Hawaiian green turtle herpesvirus]; 98% identity with gb|AAU84534.1| polymerase [Fibropapilloma-associated turtle herpesvirus]
F-UL31 c(87852..88775) [Superfamily] cl14325, nuclear egress lamina protein UL31; 99% identity with gb|AAU93329.1| nuclear phosphoprotein [Hawaiian green turtle herpesvirus]
F-UL32 c(88768..90408) 100% C-terminal identity with gb|AAU93330.1| DNA cleavage/packaging protein [Hawaiian green turtle herpesvirus
F-UL33 90371..90706 DNA cleavage/packaging protein [Hawaiian green turtle herpesvirus] (Quackenbush et al., Virology 246 (2), 392-399 (1998); 100% identity with gb|AAU93331.1| DNA cleavage/packaging protein [Hawaiian green turtle herpesvirus]
F-UL34 90719..91513 100% identity with gb|AAU93332.1| UL34 membrane-associated phosphoprotein [Hawaiian green turtle herpesvirus]
F-UL35 91561..91926 100% identity with gb|AAU93333.1| VP26 basic phosphorylated capsid protein [Hawaiian green turtle herpesvirus]
F-UL36 c(91944..98897) PFAM: [Superfamily] cl04174, Herpesvirus UL36 VP1/2 tegument protein; This family only covers a small central part of this large protein.
F-UL37 c(98897..102139) PFAM: [Superfamily] cl04350, Herpesvirus UL37 tegument protein; closest similarity to gb|AAY59063.1| small tegument protein [Tortoise herpesvirus]
F-UL38 102138..103463 PFAM: [Superfamily] cl04010, Herpesvirus capsid shell protein VP19C; closest similarity to gb|AAY59064.1| minor capsid protein [Tortoise herpesvirus]
HP25 102623..103132 Hypothetical protein with predicted bipartite NLS
F-UL39 103441..103923 Partial similarity to gb|AAQ73541.2| ribonucleotide reductase large subunit [Tortoise herpesvirus]
F-UL41 c(104719..105897) Closest similarity to gb|AER28066.1| tegument host shutoff protein [Gallid herpesvirus 1]; PFAM: cd09867, PIN domain of Flap Endonuclease-1, a structure-specific, divalent-metal-ion dependent, 5′ nuclease and homologs.
F-UL42 105949..107025 DNA polymerase processivity factor; similarity to ref|NP_944415.1| DNA polymerase processivity subunit [Psittacid herpesvirus 1];
F-UL43 107061..108287 Similarity to gb|AEI00251.1| UL43 protein [Gallid herpesvirus 3]
HP26 c(107196..107687) Hypothetical protein
HP27 108437..109090 Hypothetical protein of 217 aa with at least two Zink-binding domains identified; similarity to ref|YP_001285929.1| protein IG [Psittacid herpesvirus 1]
F-UL53 c(109136..110197) PFAM: [Superfamily] cl03284, UL53 cell fusion glycoprotein K; highest blast scores with envelope glycoprotein gK gK of psittacid herpesvirus 1, anatid-, felid1, gallid3, equid4 herpesviruses; 6 transmembrane helices predicted
F-UL52 c(110214..113087) PFAM: [PHA03180], UL52 helicase-primase primase subunit; similarity to gb|AAG30093.1|AF282130_56 DNA helicase/primase complex protein [Meleagrid herpesvirus 1]
HP28 (UL55) 113179..113805 Limited similarity to UL55 on aa level; E-value 93–04 similarity to ref|YP_443903.1| nuclear protein UL55 [Papiine herpesvirus 2]
HP29 c(113896..114384) Hypothetical protein
F-M04 c(114874..115683) Similarity to emb|CAJ84726.1| m04 protein [Murid herpesvirus 1]; m04 proteins are known to mediate immune evasion by interference with the major histocompatibility complex class I (MHC-I) pathway of antigen presentation to cytolytic T lymphocytes. Instead, the m04 early gene product binds to folded MHC-I molecules in the ER and directs the complex to the cell surface; two transmembrane helices predicted
HP30 c(116252..116788) Hypothetical protein; ORF may N-terminally extend for up to 46 aa; similarity to emb|CAC85009.1| hypothetical protein [Saimiriine herpesvirus 2]; predicted features: signal sequence, transmembrane helix; N-glycosylation site at pos. 40 NFTL
F-lec1 117032..117658 Probable start codon GGC; PFAM: cl02432 [Superfamily] cl02432, C-type lectin (CTL)/C-type lectin-like (CTLD) domain; CLECT: C-type lectin (CTL)/C-type lectin-like (CTLD) domain; PFAM: cd03593, C-type lectin-like domain (CTLD) of the type found in natural killer cell receptors (NKRs); CLECT_NK_receptors_like: C-type lectin-like domain (CTLD) of the type found in natural killer cell receptors (NKRs); transmembrane helix predicted
F-lec2 117767..118297 Alternative AAA-start codon at position −57; similar to type II membrane protein CD69 [Equus caballus]; E-value: 5e-09: “C-type lectin domain family (poxviruses)”; PFAM: cd03593, C-type lectin-like domain (CTLD) of the type found in natural killer cell receptors (NKRs); CLECT_NK_receptors_like: C-type lectin-like domain (CTLD) of the type found in natural killer cell receptors (NKRs); E value 1e-10: dbj|BAG69470.1| c-type lectin-like receptor [Gallus gallus]; transmembrane helix predicted
F-sial 118474..119433 Similarity to CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase (mus musculus); PFAM: cl02965 [Superfamily] cl02965, Glycosyltransferase family 29 (sialyltransferase) ; E value 6e-70: emb|CAB53395.1| Gal(beta)1,3/4-GlcNAc (alpha)2,3-sialyltransferase [Mesocricetus auratus]; transmembrane helix predicted
F-Nec2 c(120491..122230) Similarity to CD155 (poliovirus receptor) and CD112 (Nectin-2); E-value: 0.016 with glycoside hydrolase family 5 [Eubacterium cellulosolvens 6]; signal sequence predicted
HP31 120755..121279 Hypothetical protein with similarity to Tau-tubulin kinase 1; E-value: 0.034
HP32 c(122359..124044) Hypothetical V-set protein; pfam07686, Immunoglobulin V-set domain; Signal sequence predicted
HP33 124921..125382 Hypothetical protein
HP34 126003..126476 Hypothetical protein
HP35 c(127044..128483) PFAM: cl02885 [Superfamily] cl02885, heptad repeat 1-heptad repeat 2 region (ectodomain) of the transmembrane subunit of various endogenous retroviruses (ERVs) and infectious retroviruses; signal sequence and transmembrane helix predicted
HP36 c(128486..128938) Hypothetical protein; PFAM: [Superfamily] cl11403, Cellular and retroviral pepsin-like aspartate proteases.
HP37 (ICP4c) c(128969..131062) Hypothetical protein with limited similarity to ICP4 stretching over several domains; predicted features: several NLS, DNA- and RNA-binding domains
HP38 (ICP4b) c(130312..131055) Hypothetical protein with similarity to ICP4s over several regions; predicted features: C-terminal RNA-binding domain; mitochondrial targeting peptide