Table 5. PCR primers used for overlapping PCR.
| Designation | oligonucleotide sequence (5′ to 3′) |
| P1Fa | CGACATGCCCCAAATAATGTCG |
| P2Ra | GAATTCGTGCCTCTTGGGTAACC |
| P3F | AATTCAGTAAGCATTGTATTGT |
| P4R | GACCTGGGCTACTTGATAAGGG |
| P5R | CATTTTTATTAAGGAGGAAACC |
| P6F | TTAGATCGCCAGTTTGGTTA |
| P7F | CATCAGCCTTTGCGTATTT |
| P8R | CCTTAACGCTCGAAACAATA |
| P12R | CATCAGACTCCAAAACCAAA |
| P13F | GAGGAAAGATAGGCGCAAA |
| P16R | GGGGACCACTTTGTACAAGAAAGCTGGGTTTACGTTAGGCAGACGTTC b |
| P17F | GGTTTCCTCCTTAATAAAAATG |
| P18R | TTTGCGCCTATCTTTCCTC |
| P19F | AGGTAACAGGTTTTGCGAAG |
a
= PCR primers (F = forward; R = reverse)
b
= this primer consists of a binding part (bold) and is fused to a 5′-att-recombination sequence. The latter part of the primer was not required for the present experiment but was, nevertheless, part of the primer.