Table 6. PCR primers used for detection of F-M04- and F-sial RNA^a.

Designationb	oligonucleotide sequence (5′ to 3′) c
Att-SiaF	GGGGACAAGTTTGTACAAAAAAGCAGGCTATGAGGCGCAATGTAAAA
P16R	GGGGACCACTTTGTACAAGAAAGCTGGGTTTACGTTAGGCAGACGTTC
Att-M04F	GGGGACAAGTTTGTACAAAAAAGCAGGCTATGAAAAGATACTCGTTC
M04R-att	GGGGACCACTTTGTACAAGAAAGCTGGGTTTTGAATTAAATACAAATC

^a = these primers were also used to amplify and clone (for sequence determination) the Australian isotypes of F-M04 and F-sial

^b = PCR primers (F = forward; R = reverse)

^c = each primer consists of a binding part (bold) and is fused to a 5′-att-recombination sequence. The latter part of the primer was used for consecutive cloning and sequencing of the amplification product into a Gateway donor vector.