Figure 2. The binding affinity assay of the enriched pools with U118 MG and SVGp12 cells.

(A) The fluorescence intensity of U118-MG cells binding with selected pools increased gradually as the selection progressed, indicating that the enhanced binding affinity of enriched pools. (B)The selected pools also showed increased affinity with SVGp12 cells, but the response was apparently weaker than with U118-MG cells. Final concentration of FAM-labeled ssDNA pools was 250 nM in binding buffer.