Figure 4. Confocal images of cultured U118-MG and SVGp12 cells staining with FAM-labeled aptamers.

Cells were incubated with FAM labeled initial library and aptamers. The initial library showed the background binding and aptamers showed significant binding over the background. For U118-MG cells: A =  initial library; B =  GBM128; C =  GBM131. For SVGp12 cells; D =  initial library; E =  GBM128; F =  GBM131. The final concentration of FAM-labeled aptamers was 250 nM.