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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Oral Oncol. 2012 Jun 23;48(11):1136–1145. doi: 10.1016/j.oraloncology.2012.05.015

Figure 4. Concordance of assay platforms.

Figure 4

Sixty-six patient serum samples of two analytes IL-6 and HGF were measured by ELISA, Luminex and Searchlight. ELISA was regarded as the reference standard and plotted on the X axis of all plots. A linear regression model was fit for each pair with Luminex (left column) or Searchlight (right column) serving as the dependent variable. Based on examination of residuals either log or square root transformations were required for selection of correct models. The solid lines are predicted values from the best fitting regression models. A dashed diagonal reference has been added to show hypothetical perfect agreement. When compared to ELISA, the Luminex assay has a restricted linear range and consistently underestimates analyte expression.