Skip to main content
. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Alcohol Clin Exp Res. 2012 Apr 6;36(11):1873–1881. doi: 10.1111/j.1530-0277.2012.01787.x

Fig. 5.

Fig. 5

The effect of chronic ethanol (EtOH) exposure on aryl hydrocarbon receptor (AhR), cytochrome P450 (CYP) 1A1, and CYPB1 expression. Mouse hepatic stellate cells (MHSCs) were incubated with culture medium alone (control), 50 mM EtOH for 7 days (50 mM/7 d), or 50 mM EtOH for 7 days followed by 200 mM EtOH treatment for another 6 hours (50 mM/7 d + 200 mM/6 h). The level of AhR protein was determined by Western blot analysis and expressed relative to β-actin. (A–B) The level of AhR mRNA was determined by quantitative real-time reverse transcription polymerase chain reaction (RT-PCR) and normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and AhR protein by Western blot analysis and expressed relative to β-actin. (C–D) CYP1A1 and 1B1 mRNAs were determined by RT-PCR, normalized to GAPDH. Values represent the mean ± SEM of 5 independent experiments. *p < 0.05 versus cells without EtOH treatment (control), †p < 0.05 versus cells treated with 50 mM EtOH alone for 7 days.