. 2012 May 24;350(1):157–166. doi: 10.1007/s00441-012-1431-7

Table 1.

Sequences of oligonucleotide primers used for RT-PCR and qRT-PCR analyses

Gene	Primers (5' → 3')	Product size	Reference
VEGF-A all isoforms^a	Forward: TGCGGATCAAACCTCACCAAA	125–380 bp	Tsoi et al. (2002)
VEGF-A all isoforms^a	Reverse: TCACCGCCTCGGCTTGTCACA	125–380 bp	Tsoi et al. (2002)
VEGF-A ₁₂₀	Forward: AAGGCCAGCACATAGGAGAG	101 bp	Kaczmarek et al. (2008)
VEGF-A ₁₂₀	Reverse: CCTCGGCTTGTCACATTTTT	101 bp	Kaczmarek et al. (2008)
VEGF-A ₁₆₄	Forward: GAGGCAAGAAAATCCCTGTG	150 bp	Kaczmarek et al. (2008)
VEGF-A ₁₆₄	Reverse: TCACATCTGCAAGTACGTTCG	150 bp	Kaczmarek et al. (2008)
Flt-1 VEGF receptor 1	Forward: TGGATTTCAGGTGAGCTTGGA	68 bp	Redmer et al. (2005)
Flt-1 VEGF receptor 1	Reverse: TCACCGTGCAAGACAGCTTC	68 bp	Redmer et al. (2005)
KDR VEGF receptor 2	Forward: CTTCCAGTGGGCTGATGACC	67 bp	Redmer et al. (2005)
KDR VEGF receptor 2	Reverse: GCAACAAACGGCTTTTCATGT	67 bp	Redmer et al. (2005)
NRP-1 neuropilin	Forward: GATTGCGGTGGACGATATTAGC	60 bp	Vonnahme et al. (2006)
NRP-1 neuropilin	Reverse: GGTTTTGCGCAGTCCTCTTG	60 bp	Vonnahme et al. (2006)
PPIC peptidyl-prolyl cis-trans isomerase C	Forward: TGGCACTGGTGGTATAAGCA	145 bp	Herman et al. (2010)
PPIC peptidyl-prolyl cis-trans isomerase C	Reverse: GGGCTTGGTCAAGGTGATAA	145 bp	Herman et al. (2010)

^aPrimers used in RT-PCR analysis, the expected sizes for ovine VEGF205, 188, 164, 145 and 120 are 380, 329, 257, 198 and 125 bp, respectively.