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. Author manuscript; available in PMC: 2012 Oct 3.
Published in final edited form as: J Biol Chem. 1992 May 25;267(15):10764–10770.

FIG. 5. Representative experiment demonstrating R-PIA-mediated inhibition of forskolin-stimulated adenylylcyclase activity in membranes prepared from CHO cells stably expressing bovine brain A1AR cDNA.

FIG. 5

Assays were performed as described under “Experimental Procedures” using ~100 μg of protein per assay tube. Forskolin was present at 50 μM where indicated and the concentration of R-PIA was as shown. This experiment was performed four times with similar results.