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. Author manuscript; available in PMC: 2012 Oct 3.
Published in final edited form as: J Biol Chem. 1992 May 25;267(15):10764–10770.

FIG. 8. Representative [125I]APNEA saturation assay in membranes prepared from COS7 cells transiently expressing wild-type and mutagenized (His-251 → Leu-251) A1AR cDNA.

FIG. 8

Nonspecific binding was defined with 10 mM theophylline. The best-fit lines for nonspecific binding for both groups were superimposable. Approximately equivalent amounts (~3.5 μg) of protein were used per assay tube for both groups. Other experimental details are described under “Experimental Procedures.” This experiment was repeated three times.