Figure 2.

Detection of FLAG-tagged isoforms of Nitr9 from transfected cells by indirect immunofluorescence. HEK293T cells were transfected with plasmids encoding FLAG-tagged Nitr9 isoforms and EGFP as indicated on top of the panels. FLAG-tagged Nitr9 proteins were detected with (a) an anti-FLAG antibody, (b) anti-Nitr9⁹⁰ or (c) anti-Nitr9¹⁹, and a PE conjugated secondary antibody (red). Transfected cells can be identified by EGFP expression (green). DAPI labels the nuclei of all cells (blue). The pIRES2-EGFP parental plasmid was included as a negative control.