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Detection of endogenous isoforms of Nitr9 from transfected cells by indirect immunofluorescence. HEK293T cells were transfected with plasmids encoding endogenous isoforms of Nitr9 and EGFP as indicated on top of the panels. Nitr9 proteins were detected with (a) anti-Nitr990 or (b) anti-Nitr919 and a PE conjugated secondary antibody (red). Transfected cells can be identified by EGFP expression (green). DAPI labels the nuclei of all cells (blue).
