Fig. 2.

Regulation of MMP-2 activity by components of CNSE. A, zymogram showing MMP-2 activity of conditioned media from 3T3-L1 fibroblast cells treated with 0.5% DMSO (lane 1), quercetin at 50 μg/ml (lane 2), commercial anacardic acid (AA) (Calbiochem) (lane 3), the cardol-cardanol mixture (lane 4), CNSE (lane 5), and anacardic acid isolated form CNSE (lane 6), each at 1 μg/ml. B, a representative plot of percentage inhibition observed in the zymogram. Each bar represents the mean ± S.E. of triplicate determinations from three independent experiments. ***, P < 0.001 (one-way analysis of variance with Dunnett's multiple-comparison post-test). CTL, control.