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. 2012 Oct;82(4):679–686. doi: 10.1124/mol.112.080085

Fig. 2.

Fig. 2.

Cross-reactivity of anti-CYP4V2 IgG with other CYP4 enzymes and CYP4V2 protein expression in ARPE-19 cell lysate. The anti-CYP4V2 antibody specifically reacted with CYP4V2. a, lanes 1, 5, and 9, 0.2 pmol of CYP4V2; lane 2, 1 pmol of rabbit CYP4B1; lane 3, 1 pmol of CYP4A11; lane 4, 1 pmol of CYP4F2; lane 6, 1 pmol of CYP4F3A; lane 7, 1 pmol of CYP4F3B; lane 8, 1 pmol of CYP4F12. b, left lanes, 330, 160, and 82 fmol of purified CYP4V2; right lanes, 10 and 30 μg of ARPE-19 cell lysate. CYP4V2 was expressed at a concentration of ∼6 pmol/mg of cell lysate. Band intensities were analyzed with an Odyssey system.