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Characterization of purified His6-CYP4V2 through SDS-PAGE and UV spectrometric analyses. a, purified CYP4V2 migrated with an apparent molecular mass of ∼60 kDa. b, the absolute UV spectrum of CYP4V2 exhibited a Soret band at 419 nm and α and β bands at 571 and 535 nm. c, the reduced carbon monoxide-bound difference spectrum of purified CYP4V2 demonstrated the absence of cytochrome P420.
