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. 2012 Oct;82(4):679–686. doi: 10.1124/mol.112.080085

Fig. 6.

Fig. 6.

Expression and functional consequences of the p.H331P variant of CYP4V2 stably transduced in HepG2 cells. a, real-time RT-PCR analysis of CYP4V2 wild-type and p.H331P mRNA in HepG2 cells. b, Western blot analysis of CYP4V2 wild-type and p.H133P protein in HepG2 cells. β-Actin was detected as a loading control. c, DHA, EPA, and arachidonic acid accumulation in p.H331P-transfected cells, compared with cells expressing the wild-type enzyme. Data are the mean and S.D. of triplicate experiments. WT, wild-type; FA, fatty acids. *, p < 0.02.