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. 2012 Oct;82(4):601–613. doi: 10.1124/mol.112.078147

Fig. 5.

Fig. 5.

The role of MAPKs in transactivation of Aldh1a1 promoter activity. Aldh1a1 transcriptional activity is significantly reduced by inhibition of JNK (SP600125) and MEK (PD98059) kinase and increased by inhibition of protein phosphatase 2A [okadaic acid (OA)] activity in HepG2 cells. HepG2 cells were cotransfected with p2.0Aldh1a1Luc (−1963/+27Aldh1a1) construct and a c-Jun expression plasmid. Cells were treated with either 0.1% DMSO, 50 μM SP600125, 50 μM PD98059, or 40 nM OA for 17 h. Data are mean luciferase activities ± S.D. from at least three independent experiments and are presented as fold change over control vector cells. *, p < 0.05, significantly different compared with vector transfected cells; **, p < 0.05, significantly different from c-Jun-transfected cells (one-way ANOVA followed by the Tukey test).