TABLE 1.
Effects of Nrf2 genotype on changes in mRNA levels of antioxidant and electrophile detoxification genes in response to dietary BHA
qRT-PCR analysis of gene expression in the liver of Nrf2(+/+) and Nrf2(−/−) mice on C57BL/6 background in response to dietary administration of the phenolic antioxidant, BHA. Values are average fold change per genotype in the liver of Nrf2(+/+) and Nrf2(−/−) mice. The Nrf2(+/+) mice were fed either an AIN-76A diet (control, n = 4) or a diet supplemented with 0.45% BHA (treated, n = 4) for 7 days. The Nrf2(−/−) mice were fed chow diet only (control, n = 3) or diet supplemented with 0.45% BHA (treated, n = 3). RNA extracted from the liver was reversed-transcribed to cDNA. The mRNA levels of Aldh1a1, Aldh1a7, Aldh2, Aldh1b1, Ho-1, Nqo1, and Gstm1 gene expression were analyzed by qRT-PCR. For each sample, a single mRNA was analyzed in triplicate, and the average is shown.
| Gene | Constitutive |
Inducible |
Fold Induction |
|||
|---|---|---|---|---|---|---|
| Nrf2(+/+) | Nrf2(−/−) | Nrf2(+/+) | Nrf2(−/−) | Nrf2(+/+) | Nrf2(−/−) | |
| Aldh1a1 | 1.0 | 0.22 | 2.51 | 0.43 | 2.5* | 2.0** |
| Aldh1a7 | 1.0 | 0.38 | 2.07 | 0.66 | 2.1* | 2.2** |
| Aldh2 | 1.0 | 0.23 | 1.28 | 0.31 | 1.3 | 1.4 |
| Aldh1b1 | 1.0 | 0.53 | 1.06 | 1.29 | 1.1 | 2.4** |
| Gstm1 | 1.0 | 0.1 | 10.8 | 0.27 | 10.8* | 2.6*** |
| Nqo1 | 1.0 | 0.11 | 8.8 | 0.48 | 8.8* | 4.6*** |
| Ho-1 | 1.0 | 0.77 | 2.84 | 1.09 | 2.6* | 1.4*** |
P < 0.05, significantly increased compared with untreated Nrf2(+/+) mice (two-tailed t test).
P < 0.05, significantly increased compared with untreated Nrf2(−/−) mice.
P < 0.05 decreased fold-induction compared with Nrf2(+/+) mice.