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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Mol Cancer Res. 2012 Aug 9;10(9):1203–1215. doi: 10.1158/1541-7786.MCR-12-0124

Figure 2. NO activates EGFR via S-nitrosation post-translational modification.

Figure 2

(A) Steady-state NO concentration in DETANO-supplemented media as determined by chemiluminescence. Mean [NO] (± sem) values are shown. (B) Nitrosative ability of DETANO as determined by DAN fluorescence assay. Mean DAN-triazole fluorescence (RFU) values (± sd) are shown and linear regression analysis (± 95% CI) indicates a linear response between DETANO concentration and nitrosative capacity. (C) Total cellular protein S-nitrosation from MDA-MB-468 cells treated with DETANO for 24 hours compared to total cellular protein content. (D) Total protein, immunoprecipitated EGFR and EGFR-SNO from MDA-MB-468 cells treated with DETANO for 24 hours. (E) Western blot of relative EGFR tyrosine 1173 phosphorylation and EGFR-SNO modification from MDA-MB-468 cells treated with EGF (10 ng/mL) or DETANO and chemical inhibitors of S-nitrosation.