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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Mol Cancer Res. 2012 Aug 9;10(9):1203–1215. doi: 10.1158/1541-7786.MCR-12-0124

Figure 6. NO increases cellular EMT.

Figure 6

(A) Representative microphotographs of MDA-MB-468 cells compared to cells exposed to 0.5 mM DETANO showing altered cellular morphology and cell-to-cell adhesion. (B) Representative western blot of E-cadherin and vimentin expression in MDA-MB-468 cells exposed to 0.5 mM DETANO alone or with GSH, N3− or PP2. (C) Cellular proliferation in serum-starved MDA-MB-468 or SKBR3 cells exposed to EGF or 0.5 mM DETANO. Significance from control was determined by one-way ANOVA with Dunnett’s post-test analysis (*P < 0.01). (D) Western blot of relative COX-2 expression in MDA-MB-468 cells treated with DETANO for 24 hours as compared to actin. (E) COX-2 activity as determined by PGE2 measurements in DETANO-treated MDA-MB-468 conditioned media. Data represent mean PGE2 concentrations (± sd). Significance from control was determined by one-way ANOVA with Dunnett’s post-test analysis (*P < 0.05).