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. Author manuscript; available in PMC: 2013 Aug 16.
Published in final edited form as: J Phys Chem B. 2012 Aug 1;116(32):9627–9634. doi: 10.1021/jp304613b

FIG. 5.

FIG. 5

Temperature-dependent difference spectra of A57-labeled HP36. Difference spectrum are calculated by subtracting the spectrum at 7 °C from the higher-temperature spectra. (a) Experimental spectra from 7 °C to 86 °C in ~ 10 °C increments (reprinted with permission from Biochemistry 46, 3279 (2007); copyright 2007 American Chemical Society).72 (b) Theoretical data for the spectral density from 7 °C to 146 °C in ~ 20 °C increment. The absolute intensities in experiment and theory are in arbitrary units, and therefore are not directly comparable.