Table 2.
Responses of RCA culture to various supplements
| Treatment | Rosette colonies | Interpretation |
| Sea water | − | |
| CM from ATCC50818 | + | RIF-1 present in environmental isolate ATCC 50818 |
| CM from RCA | − | RIF-1 is absent in RCA lines |
| Live A. machipongonensis (cell pellet) | ++ | RIF-1 is produced by A. machipongonensis |
| Heat killed A. machipongonensis (cell pellet) | ++ | RIF-1 is resistant to heat |
| A. machipongonensis CM | + | RIF-1 is released by live Algoriphagus |
| A. machipongonensis CM, boiled 10 min | + | RIF-1 is not labile |
| A. machipongonensis CM + Proteinase K | + | RIF-1 is not a protein |
| A. machipongonensis CM + DNAse | + | RIF-1 is not DNA |
| A. machipongonensis CM + RNAse | + | RIF-1 is not RNA |
| A. machipongonensis CM, MeOH extract | + | RIF-1 is an organic compound |
| A. machipongonensis cell pellet, MeOH extract | ++ | RIF-1 is present in the Algoriphagus cell envelope |
| A. machipongonensis cell pellet, Bligh-Dyer extract | ++ | RIF-1 is a lipid |
| Sphingomyelin (20 mg mL−1) | − | Sphingomyelin does not induce rosette colony development |
| Monosialoganglioside (20 mg mL−1) | − | Monosialoganglioside does not induce rosette colony development |
| Galactocerebroside (20 mg mL−1) | − | Galactocerebroside does not induce rosette colony development |
| N-palmitoyl-DL-dihydrolacto cerebroside (20 mg mL−1) | − | N-palmitoyl-DL-dihydrolacto cerebroside does not induce rosette colony development |
−: no induction; +: low induction; ++: high induction.
CM: conditioned medium; RCA: rosette colonies absent; RIF-1: rosette inducing factor 1.