Figure 2.

PICK1 enhances TβRI degradation. (A) MEFs were harvested for immunoblotting. Tubulin served as a loading control. (B) MEFs were treated with cycloheximide (CHX) for the indicated time and harvested for immunoblotting. The band intensity was quantitated and the statistical analysis of 3 independent experiments was provided. (C) HEK293T cells transfected with TβRI and PICK1 as indicated were treated with NH₄Cl (25 mM), chloroquine (100 μM) or MG132 (25 μM) for 4 h before harvested for immunoblotting. ^* indicates a nonspecific band. (D) HEK293T cells transfected with the indicated constructs were treated with chloroquine and MG132 and harvested for Ni-nitrilotriacetate bead precipitation, followed by immunoblotting.