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. 2012 Jun 5;22(10):1453–1466. doi: 10.1038/cr.2012.88

Figure 3.

Figure 3

p53 and p21 are the downstream targets of CFTR-HCO3−-miR-125b pathway. (A) Immunofluorescent results showing increased expression of p53 (green) and p21 (red) in HCO3−-free condition or after CFTRinh172 treatment (5-10 embryos/group). (B) Western blot results showing increased expression of p53 and p21 in two-cell embryos from Cftr+/− mice (H × H) compared to those from Cftr+/+ (W × W) mice. (C) Western blot results showing upregulation of p53 and p21 by CFTRinh172 or HCO3−-free condition in two-cell embryos. (D) Western blot results showing that transfection of anti-miR-125b oligo leads to increased expression of p53 and p21, while transfection of miR-125b precursor leads to decreased expression of p53 and p21 in two-cell embryos. Scale bar, 50 μm.