FIG. 3.

Light microscopy of myoblast clusters (indicated by white arrows) growing in the scaffold pores 2 weeks after seeding (a). Scaffold area is outlined with dashed white lines. Cells both migrated out of the scaffold 2 weeks after they were initially seeded (b, c), and continued to grow (cells indicated with white arrow heads) on the plate surface (d). About 0.3×10⁶ PMMGFP cells were initially seeded on the 5% 1LMW:1HMW scaffolds and maintained under cell culture conditions as described in the Methods. Images are taken at 200× with a Nikon e600 microscope equipped with a DP70 Camera. Color images available online at www.liebertpub.com/tea