FIG. 1.

Sequential electrospinning of poly(ɛ-caprolactone) at varying concentrations and collecting conditions was performed to generate dense fiber networks that preserve zonal fiber mechanical properties in a continuous scaffold. The diagram (A) illustrates the zonal placement of varying fiber zones and the resultant electron microscopy images of the bulk anisotropic scaffold (B) and the varying fibers of aligned 1-μm (C), randomly oriented 1-μm (D), or randomly oriented 5-μm fibers throughout the scaffold (E). Scale bar=100 μm in (B) and 10 μm in (C–E).