FIG. 4.

Histological staining of extracellular matrix deposition in fibrous scaffold of aligned (A, B), randomly oriented (C, D), and trilaminar composite scaffolds (E, F) after 5 weeks in culture. Fibrous scaffolds were stained with Picrosirius Red for collagen content (A, C, E) or Alcian Blue for GAG content (B, D, F). Aligned and randomly oriented fibrous scaffolds exhibited homogenous extracellular matrix deposition, whereas in trilaminar composites, matrix deposition was zone specific. Scale bar=500 μm.