FIG. 6.

Histological analysis of the neocartilage with neoperichondrium 6 months after transplantation. (a) Hematoxylin and eosin and (b) toluidine blue staining shows that neocartilage produced a large amount of aggrecan. (c) Elastica Van Gieson staining showed that neocartilage contained elastic fibers. Immunohistochemistry revealed that elastic chondrocytes regenerated neocartilage-containing type II collagen (d) and neoperichondrium-containing type I collagen (e). (f, g) Normal human auricular elastic cartilage-containing type I or type II collagen, positive control. Vessels were seen in both normal perichondrium and neoperichondrium. Also, the type I collagen was seen rich in the neocartilage matrix than in the auricular cartilage matrix.