TABLE 4.
Fluorescent-activated cell sorting analysis of CaVβ3 constructs
FACS results obtained after the transient transfection of CaV2.3-HA WT in HEKT cells. The results are reported as percentage values of cells in the higher quadrant of fluorescence. The data were pooled from experiments carried out over a period of 12 months. The data are shown as the means ± S.E. of the individual experiments, and the number of experiments (n) appears in the last column.
| CaVβ constructs |
Fluorescence |
||
|---|---|---|---|
| Transient expression HA-CaV2.3 HEKT cells | Mean | S.E. | n |
| % | % | ||
| CaVβ3 WT | 36.3 | 0.8 | 46 |
| Δ57–123 (ΔNSH3) | 26 | 1 | 6 |
| Δ57–180 (ΔSH3) | 2.9 | 0.4 | 3 |
| Δ180–364 (ΔGK) | 1.4 | 0.4 | 5 |
| Δ54–362 (ΔSH3-GK) | 3 | 1 | 3 |
| Δ170–175 (ΔPYDVVP) | 23 | 2 | 7 |
| Δ170–175 Δ180–364 (ΔPYDVVPΔGK) | 3 | 1 | 3 |
| Δ170–175 Δ57–123 (ΔPYDVVPΔNSH3) | 25 | 2 | 8 |
| Δ195–200 (ΔMMQKAL) | 1.6 | 0.6 | 5 |
| ΔHOOK (Δ122–169) | 24 | 2 | 4 |
| ΔHOOKΔPYDVVP (Δ122–175) | 22 | 1 | 4 |
| ΔPSMRPV (Δ175–180) | 3 | 0.8 | 3 |
| P175A | 33 | 1.5 | 3 |
| P175G | 32 | 2.4 | 3 |
| P179A | 29 | 0.7 | 3 |
| P179G | 30 | 0.7 | 3 |
| M195A | 37 | 0.3 | 5 |
| M196A | 22 | 0.7 | 9 |
| K198A | 41 | 0.4 | 4 |
| L200G | 18 | 0.7 | 3 |
| S295A | 37 | 1.5 | 4 |
| R302G | 43 | 1.1 | 4 |
| L303G | 8.0 | 0.2 | 7 |
| R307A | 27 | 1.1 | 3 |
| R307G | 33 | 1.6 | 3 |
| R307K | 33 | 0.6 | 3 |
| L337G | 16 | 0.7 | 6 |
| E339G | 21 | 1.3 | 3 |
| N340G | 33 | 1.0 | 3 |
| L342G | 16 | 0.5 | 6 |
| A345G | 20.2 | 0.7 | 3 |
| L200G/L303G | 1.7 | 0.1 | 3 |
| L200G/L303G/L337G | 2.1 | 0.1 | 3 |
| L200G/L303G/L337G/L342G | 2.5 | 0.1 | 3 |