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. 2012 Jul 25;287(39):33080–33093. doi: 10.1074/jbc.M112.360412

FIGURE 1.

FIGURE 1.

FBPase degradation in the vacuole requires the VPS34 gene. A and B, wild-type and cells lacking the VPS34 gene were starved of glucose for 1 day (A) and 3 days (B). Cells were transferred to medium containing high glucose for 0, 2, and 3 h and examined for FBPase degradation. C, cells lacking VPS15 and VPS30 were starved of glucose for 3 days, transferred to medium containing high glucose for 0, 2, and 3 h, and examined for FBPase degradation. D, wild-type cells expressing Vps34p-GFP were starved of glucose for 3 days, re-fed with glucose for the indicated time points, and examined for the degradation of FBPase. E, the distribution of Vps34p-GFP and actin patches was determined using fluorescence microscopy.