FIGURE 2.

Vps34p distribution is affected in cells lacking the SLA1 and ARC18 genes. A, wild-type and cells lacking the SLA1 and ARC18 genes were starved of glucose for 3 days and replenished with glucose. FBPase degradation was examined after the addition of glucose for 0, 2, and 3 h. B–D, Vps34p-GFP was expressed in the Δsla1 (B), the Δarc18 (C), and the Δvps15 (D) strains. These cells were starved of glucose for 3 days and shifted to glucose for the indicated times. The distribution of Vps34p-GFP and actin patches was determined by fluorescence microscopy.