FIGURE 4.

FBPase is in the periplasm in prolonged-starved wild-type cells. FBPase distribution was determined in wild-type cells that were grown in glucose-rich medium (A), in cells starved of glucose for 3 days (B), and in cells that were starved and then re-fed with glucose for 15 min (C) and 2 h (D). Cells were processed and embedded as described under “Experimental Procedures.” Thin sections were incubated with FBPase antibodies followed by goat anti-rabbit antibodies conjugated with 10-nm colloid gold particles and then visualized with transmission electron microscopy. Bars, 200 nm. The number of gold particles in the cytoplasmic and periplasmic space was 6 and 17 before starvation (A), 62 and 156 after 3 days of starvation (B), 260 and 16 after re-feeding for 15 min (C), and 8 and 16 after re-feeding for 120 min (D).