FIGURE 3.
Sulfs promote myoblast fusion in culture. Primary cultures of WT and SulfSK-DN myoblast cells were maintained in differentiation media without the presence of FGF2 for 48 h before assessment of myogenin expression in A and B and fusion in C and D. Nuclei were labeled by Hoechst. Myofibers were labeled with the MF20 antibody in C. Percentages of myogenin+ cell in WT and SulfSK-DN cultures were shown in B. The efficacy of fusion was quantified by percentages of mono- and multi-nucleated MF20+ myofibers in D. Data represent the mean and S.D. of three independent experiments. #, p < 0.01; **, p < 0.05; *, p < 0.1. Scale bar = 100 μm.