FIGURE 9.
Canonical and noncanonical Wnts differentially affects myoblast fusion. A, primary myoblast cells of WT mice were treated with vehicle or Wnt7a (100 ng/ml) in differentiating media for 48 h. Myoblast fusion was assessed after immunolabeling followed by quantification of relative abundance of mono- and multinucleated myofibers. B, primary myoblast cells of SulfSK-DN mice were treated with vehicle or Wnt3a (30 ng/ml) before immunolabeling and assessment of fusion. Data represent the mean and S.D. of three independent experiments. #, p < 0.01; **p < 0.05; *, p < 0.1. Scale bars, 100 μm.