FIGURE 2.

GATA1 and GATA2 bind to two GATA motifs essential for human ST2 promoter transactivation. A and B, EMSA profiles using probes −35/−6 and −70/−40, respectively. EMSA data are representative results obtained in one of three independent experiments. In vitro transcription/translation was performed using pCR-GATA1 (GATA1) or pCR-GATA2 (GATA2) as a template. −, without Ab; G1, with anti-GATA1 Ab; G2, with anti-GATA2 Ab. Specific bands corresponding to probe·GATA1 and probe·GATA2 complexes are marked with white and gray arrows, respectively. Supershifted bands corresponding to probe·GATA1·anti-GATA1 Ab complexes are marked with a white arrow with an asterisk.