Expression and functionality of aP2-HA-GSTA4 in 3T3-L1 adipocytes.
A, relative mRNA expression of GSTA4 in control (pcDNA) and overexpressing (aP2-HA-GSTA4) adipocytes normalized to TFIIE is shown. B, expression of HA-GSTA4 (anti-HA) relative to β-actin (anti-actin) in separate overexpressing cell lines is shown. C, an in vitro GS-HNE formation assay is shown (n = 3; *, p < 0.05; **, p < 0.01).