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Electrophoretic mobility of in vitro transcribed M. barkeri tRNAPyl incubated in the presence of M. barkeri PylS or Δ92PylS. Protein concentrations added to tRNAPyl are indicated at the bottom of each gel. A, electrophoretic mobility of 20 nm tRNAPyl following incubation with increasing concentrations of PylS. B, electrophoretic mobility of 0.5 μm tRNAPyl subsequent to incubation with PylS or Δ92PylS.
