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Efficacy of D. hafniense PylSn or PylSc in forming electrophoretically detectable complexes with D. hafniense tRNAPyl. Protein concentrations employed are indicated at the bottom of each lane. A, electrophoretic mobility of 17 nm tRNAPyl after incubation with indicated amounts of PylSn. B, electrophoretic mobility of 0.5 μm tRNAPyl after incubation with PylSc or PylSn.
