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Base changes made within the D. hafniense tRNAPyld-stem and T stem and loop. Targeted base positions are indicated by the boxed bases in the tRNA. Bases that directly interact with PylSc are shown with gray shading (13). The base changes that strongly affected PylSn binding to tRNA, but still showed detectable binding in EMSA, are underlined. Base changes that eliminated the tRNA ability to form an EMSA-detectable complex with PylSn are boxed.
