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. 2012 Aug 4;287(39):32338–32345. doi: 10.1074/jbc.M112.383810

FIGURE 4.

FIGURE 4.

Neural progenitor cells differentiated from TK-hESCs (3IA) are insensitive to GCV. A and B, immunostaining assay reveals the expression of neural progenitor cells (NPC) marker genes NESTIN and SOX2. C, after treatment with or without 1.0 μm GCV for 2 days, neural progenitor cells were trypsinized, stained with phosphatidylinositol (PI), and analyzed by FACS. The sample with trophic factor withdrawn for 12 h was used as a positive control for cell death. D and E, the relative mRNA levels of TK, pluripotency markers NANOG and OCT4, and the neuroepithelial markers SOX1 and PAX6 in NPCs treated with or without 1.0 μm GCV for 2 days were analyzed by real-time PCR and compared with those in TK-hESCs. Mean values are presented with S.D. (n = 3).