FIGURE 2.

Brain insulin fails to suppress Hsl activation after 3-day HFD feeding. A and B, Western blot analyses and quantification of epidydimal fat pads harvested after euglycemic pancreatic clamps (n ≥ 5/group). A, quantification of WAT Hsl phosphorylation. B, Western blot and quantification of WAT Akt signaling. C and D, WAT (C) and liver (D) insulin signaling as assessed by Western blot analysis. Epidydimal fat and liver samples were harvested 15 min after an intraperitoneal (IP) bolus of insulin or saline. Before the intraperitoneal injection of insulin, RC- and HFD-fed rats were infused with MBH insulin or vehicle for 6 h (n = 3/group; n = 2/group for saline injected controls). N.S., not statistically significant, as indicated by brackets. E, insulin levels at base line and during the clamp (n ≥ 4/group). F and G, correlation of caloric intake on HFD and Ra glycerol at base line (F) and during the clamp period (G) (both n = 11). All error bars represent S.E.