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. 2012 Jul 31;287(39):33014–33025. doi: 10.1074/jbc.M112.389148

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — CNK3 significantly stimulates interaction of SGK1 with its target substrate, Nedd4-2. A, co-immunoprecipitation assays in transiently transfected (as specified) HEK 293T cells demonstrating that CNK3 stimulates the interaction of SGK1 with Nedd4-2. Blots showing total cellular expression of the various proteins specified are also depicted. GAPDH was used as a loading control. IB, immunoblot; WCL, whole-cell lysate. Also shown is a graphical representation of the above data (B) clearly demonstrating that CNK3 markedly augments the SGK1-Nedd4-2 interaction. ***, p < 0.001 (n = 4). Error bars represent S.E.