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. 2012 Jul 31;287(39):33014–33025. doi: 10.1074/jbc.M112.389148

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — The PDZ domain in CNK3 is essential for its ability to interact with ENaC and the key ENaC-regulatory kinase, SGK1. HEK 293T cells were transiently transfected with FLAG-tagged α-, β-, and γ-mENaC subunits and V5-tagged WT- or ΔPDZ-CNK3 with or without SGK1 as specified. Shown are co-immunoprecipitation assays demonstrating the drastically reduced ability of ΔPDZ-CNK3 to interact with ENaC (A) (n = 4) or SGK1 (B) (n = 5). Blots showing total cellular expression of the various proteins specified are also depicted. GAPDH was used as a loading control. IB, immunoblot; WCL, whole-cell lysate.