Figure 5.

Cytotoxicity analysis of silica-coated magnetic nanoparticles (SiO₂-MNP) by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) (A) and lactate dehydrogenase (LDH) (B) assays. (A) 3T3 cells were contacted with SiO₂-MNP at indicated concentration for 3 days before measuring mitochondria activity by MTT assay at 570 nm (OD₅₇₀). (B) 3T3 cells were contacted with SiO₂-MNP at indicated concentration for 1 day before measuring released LDH concentration at 490 nm (OD₄₉₀).

Note: 3T3 cells without contacting SiO₂-MNP were used as the control.