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K. pneumoniaeC3091-derived GalET confer decreased sensitivity to bile salts toE. coliEPI100. EPI100 carrying either pACYC184-galET or the pACYC184 vector control were grown for 18 hrs in LB broth in the presence and absence of increasing concentrations of bile salts after which colonisation was quantified from plating. The data are expressed as the mean ± SEM for triplicate samples. ***, p < 0.001; **, p < 0.01, as compared to untreated EPI100 vector control.
