Table 4.
Lipid metabolism genes differentially expressed in the liver of fish having high or low flesh n-3 LC-PUFA contents
|
Probe name |
Gene |
High/Low LC-PUFA |
p- value |
|
|---|---|---|---|---|
| L Lipid (LH/LL) | H Lipid (HH/HL) | |||
|
Cholesterol biosynthesis, metabolism and regulation | ||||
| Omy#S15288895 |
Sterol-C4-methyl oxidase-like |
1.84 |
1.32 |
0.0033 |
| Ssa#STIR00031_3 |
7-dehydrocholesterol reductase (7dchr) |
- 1.55 |
- 1.48 |
0.0036 |
| Ssa#S30286041 |
7-dehydrocholesterol reductase (7dchr) |
- 1.48 |
- 1.42 |
0.0120 |
| Omy#CF752841 |
Sterol regulatory element-binding transcription factor 2 (srebp2) |
- 1.71 |
- 1.31 |
0.0170 |
| Ssa#TC102141 |
Cytochrome P450, family 27, subfamily A, polypeptide 1 |
- 3.97 |
- 1.04 |
0.0192 |
| Ssa#STIR16974 |
7-dehydrocholesterol reductase (7dchr) |
- 1.46 |
- 1.31 |
0.0195 |
| Ssa#AM402497 |
Hydroxymethylglutaryl-CoA synthase 1 |
- 2.39 |
1.21 |
0.0199 |
| Ssa#STIR00031_4 |
7-dehydrocholesterol reductase (7dchr) |
- 1.41 |
- 1.46 |
0.0212 |
| Ssa#STIR00098_4 |
Isopentenyl-diphosphate delta isomerise (ipi) |
- 2.12 |
- 1.15 |
0.0228 |
| Ssa#S18867829 |
7-dehydrocholesterol reductase (7dchr) |
- 1.33 |
- 1.25 |
0.0228 |
| Ssa#DY741343 |
Lanosterol 14-alpha demethylase |
- 1.42 |
- 1.28 |
0.0346 |
| Omy#S22913656 |
Acetoacetyl-CoA synthetase |
- 1.77 |
- 2.52 |
0.0358 |
| Ssa#STIR00033_3 |
Mevalonate kinase (mev) |
- 1.54 |
- 1.08 |
0.0393 |
| Ssa#CA064135 |
Vigilin |
2.80 |
- 1.22 |
0.0423 |
| Ssa#DW582478 |
Cytochrome P450, family 8, subfamily B, polypeptide 1 |
- 1.26 |
- 1.50 |
0.0449 |
| Ssa#STIR00102_3 |
Squalene epoxidase |
- 2.11 |
- 1.36 |
0.0468 |
|
Glycerophospholipid synthesis | ||||
| Ssa#STIR39152_S |
Lipid phosphate phosphohydrolase 2 (lpp2) |
1.20 |
1.19 |
0.0177 |
| Ssa#KSS4003 |
Chka protein |
1.18 |
1.53 |
0.0264 |
| Ssa#S35538062_S |
Monoacylglycerol O-acyltransferase 1 (mgat) |
1.20 |
1.51 |
0.0303 |
| Ssa#S31963704 |
Diacylglycerol O-acyltransferase homolog 2 |
1.41 |
1.02 |
0.0358 |
| Ssa#S48418830 |
Phosphatidylglycerophosphate synthase 1 |
- 1.52 |
- 1.72 |
0.0369 |
|
Fatty acid synthesis | ||||
| Ssa#KSS4155 |
Trans-2-enoyl-CoA reductase, mitochondrial precursor |
- 1.26 |
- 1.25 |
0.0440 |
|
Intracellular fatty acid transport | ||||
| Ssa#S35585414 |
Acyl-CoA-binding protein |
10.47 |
1.65 |
0.0081 |
| Ssa#DY703528 |
Fatty acid-binding protein, intestinal |
- 1.25 |
- 1.46 |
0.0331 |
| Ssa#CB509140 |
Fatty acid-binding protein, intestinal |
- 1.20 |
- 1.35 |
0.0375 |
| Ssa#STIR04578 |
Fatty acid-binding protein, heart |
1.22 |
1.47 |
0.0401 |
|
Lipid transport | ||||
| Ssa#CK898816 |
Low density lipoprotein receptor-related protein 1 (lrp1) |
- 1.14 |
- 1.39 |
0.0341 |
| Ssa#S32008850 |
Apolipoprotein A-IV precursor (apoa4a) |
- 1.43 |
- 1.01 |
0.0377 |
| Omy#BX318293 |
Low density lipoprotein receptor-related protein 1 (lrp1) |
- 3.61 |
- 1.67 |
0.0404 |
| Ssa#S18866963 |
Apolipoprotein A-IV precursor (apoa4b) |
- 1.39 |
- 1.43 |
0.0428 |
|
Lipid hydrolysis | ||||
| Ssa#S18887340 |
Acyl-coenzyme A thioesterase 5 |
1.64 |
1.18 |
0.0007 |
| Ssa#STIR02708 |
Isoamyl acetate-hydrolyzing esterase 1 homolog |
1.54 |
1.12 |
0.0013 |
| Ssa#DW007099 |
Similar to patatin-like phospholipase domain containing 7 |
- 3.75 |
- 2.37 |
0.0070 |
| Ssa#S31963297 |
Acyl-CoA thioesterase 11 |
1.35 |
1.38 |
0.0097 |
| Ssa#STIR07750 |
N-acylsphingosine amidohydrolase 1 |
- 1.14 |
- 1.34 |
0.0122 |
| Ssa#STIR05034 |
Isoamyl acetate-hydrolyzing esterase 1 homolog |
1.74 |
1.01 |
0.0208 |
| Omy#CA366823 |
Acyl-coenzyme A thioesterase 3 |
1.33 |
2.17 |
0.0248 |
| Ssa#STIR22551 |
Lipoprotein lipase (lpl) |
1.31 |
1.31 |
0.0346 |
|
Eicosanoid synthesis and metabolism | ||||
| Ssa#TC68569 |
Thromboxane-A synthase (thas) |
- 1.33 |
- 1.67 |
0.0269 |
| Ssa#TC110080 |
Phospholipase A2 (pla2g4) |
1.26 |
1.08 |
0.0296 |
| Omy#TC147730 |
Prostaglandin I2 (prostacyclin) synthase (ptgis) |
- 2.53 |
- 4.25 |
0.0349 |
| Ssa#S35581706 |
15-hydroxyprostaglandin dehydrogenase |
- 1.51 |
- 1.09 |
0.0400 |
| Ssa#EG930234 |
Arachidonate 5-lipoxygenase (alox5) |
1.91 |
1.55 |
0.0449 |
|
Regulation of lipid metabolism | ||||
| Ssa#TC112002 |
Retinoid X receptor beta |
1.00 |
- 19.09 |
0.0000 |
| Ssa#KSS2129 |
Adiponectin receptor protein 1 |
- 1.30 |
- 1.31 |
0.0098 |
| Ssa#CA056493 |
Angiopoietin-like 6 |
- 1.63 |
- 1.07 |
0.0290 |
| Ssa#S35490606 |
Adiponectin, C1Q and collagen domain containing, like |
1.34 |
1.66 |
0.0441 |
| Ssa#S18888608 | Adiponectin receptor protein 1 | - 1.38 | - 1.40 | 0.0451 |
Results were obtained by two-way ANOVA analysis (p < 0.05; fold change cut-off of 1.2) without multiple testing correction (n = 1951 total features). Transcripts are arranged by functional categories and, within these, by p-value. Indicated are also the probe names and the expression ratio between families determined separately for each total lipid level.