Table 5.

Validation of microarray results and expression of genes of interest in relation to the factor 'n-3 LC-PUFA level'

Gene	Low Lipid LH/LL		High Lipid HH/HL
	Microarray	RT-qPCR	Microarray	RT-qPCR
LC-PUFA biosynthesis
Δ5fad		−1.19		1.17
Δ6fad		1.13		−1.21
elovl2		1.14		1.06
Cholesterol biosynthesis
ipi	−2.13	−3.92	−1.15	1.32
mev	−1.54	−1.51	−1.08	1.06
7dchr	−1.33 to −1.54	−1.47	−1.25 to −1.47	−1.34
srebp2	−1.72	−1.68	−1.30	1.60
Lipid transport and lipoprotein metabolism
lrp1	−1.14 to −3.57	−1.36	−1.39 to −1.67	1.24
apoA4a	−1.43	−1.09	1.00	1.32
apoA4b	−1.39	−1.48	−1.43	−1.10
lpl	1.31	1.23	1.31	1.38
Glycerophospholipid synthesis
lpp2	1.20	−1.19	1.19	1.30
mgat	1.20	1.04	1.51	1.78
Eicosanoid biosynthesis
alox5	1.91	1.48	1.55	1.62
pla2g4	1.26	−1.08	1.08	1.06
thas	−1.33	−1.08	−1.67	1.34
ptgis	−2.53	−1.25	−4.25	1.27
Immune response
mal	−22.20	−3.70	−25.00	−5.00
ccl13	4.40	5.98	3.00	2.14
trim25	5.20	2.80	6.70	2.51
lect2	4.60	1.92	7.10	7.57

Values represent the expression ratios between high PUFA / low PUFA, for fish containing either low or high total lipid levels in their flesh, obtained by microarray analysis or RT-qPCR. Expression ratios in bold were significant by REST2008 analysis of RT-qPCR results.

Delta5 and 6 fatty acyl desaturases (Δ5fad and Δ6fad); fatty acyl elongase (elovl2); isopentenyl-diphosphate isomerase (ipi); mevalonate kinase (mev); 7-dehydrocholesterol reductase (7dchr); sterol regulatory element-binding protein 2 (srebp2); low density lipoprotein receptor-related protein 1 (lrp1); apolipoprotein A-IV (apoa4a and apoa4b); lipoprotein lipase (lpl); lipid phosphate phosphohydrolase 2 (lpp2); monoacylglycerol O-acyltransferase 1 (mgat); arachidonate 5-lipoxygenase (alox5); phospholipase A2 (pla2g4); thromboxane-A synthase (thas); prostaglandin I2 (prostacyclin) synthase (ptgis); myelin and lymphocyte protein (mal); c-c motif chemokine 13 precursor (ccl13); tripartite motif-containing protein 25 (trim25); leukocyte cell-derived chemotaxin 2 precursor (lect2).