Table 6.
Validation of microarray results and expression of genes of interest in relation to the factor 'Lipid level'
|
Gene |
Low n-3 LC-PUFA HL/LL |
High n-3 LC-PUFA HH/LH |
||
|---|---|---|---|---|
| Microarray | RT-qPCR | Microarray | RT-qPCR | |
|
LC-PUFA biosynthesis | ||||
|
Δ5fad |
|
−1.03 |
|
1.35 |
|
Δ6fad |
|
1.04 |
|
−1.32 |
|
elovl2 |
−1.28 to −1.41 |
−1.51 |
−1.82 to −1.97 |
−1.62 |
|
Cholesterol biosynthesis |
|
|
||
|
ipi |
|
−3.95 |
|
1.31 |
|
mev |
|
−1.40 |
|
1.14 |
|
7dchr |
|
−1.01 |
|
1.09 |
|
srebp2 |
|
−2.09 |
|
1.29 |
|
Lipid transport and lipoprotein metabolism |
|
|||
|
lrp1 |
|
−1.82 |
|
−1.08 |
|
apoA4a |
|
−1.37 |
|
−0.95 |
|
apoA4b |
|
−2.43 |
|
−1.80 |
|
lpl |
|
−1.18 |
|
−1.05 |
|
Immune response | ||||
|
cfh |
−1.33 to −1.56 |
−1.24 |
−1.56 to −1.69 |
−1.31 |
|
trim25 |
−5.64 |
−2.09 |
−4.34 |
−2.33 |
| lect2 | 3.16 | 1.23 | 4.86 | 4.84 |
Values represent the expression ratios between high lipid / low lipid, for fish containing either low or high n-3 LC-PUFA levels in their flesh, obtained by microarray analysis or RT-qPCR. Expression ratios in bold were significant by REST2008 analysis of RT-qPCR results.
Delta5 and 6 fatty acyl desaturases (Δ5fad and Δ6fad); fatty acyl elongase (elovl2); isopentenyl-diphosphate isomerase (ipi); mevalonate kinase (mev); 7-dehydrocholesterol reductase (7dchr); sterol regulatory element-binding protein 2 (srebp2); low density lipoprotein receptor-related protein 1 (lrp1); apolipoprotein A-IV (apoa4a and apoa4b); lipoprotein lipase (lpl); complement factor H precursor (cfh); tripartite motif-containing protein 25 (trim25); leukocyte cell-derived chemotaxin 2 precursor (lect2).