Figure 3.

Epitope mapping of peptide 6 with eight different 10-mer peptides. Five amino acids sequences from the HPV16 L1-derived sequence at position 295 to 299 were added to the N-terminal of the first 10-mer, and those at position 320 to 324 were added to the C-terminal of the eighth 10-mer (left side of the figure). Each of these eight peptides shares seven amino acids sequences with each other. We also addressed the reactivity to immunized sera of the 9-mer peptide (qifnkpywl) at positions 305 to 313, which had binding motifs to HLA-A2 and HLA-A24. Representative results are given in the right side of the figure. The highest reactivity to one 10-mer (aqifnkpywl at position 304 to 313) is shown (^*P < 0.05 to any others tested). Wilcoxon rank sum test was used for this analysis.