Table 2.
HPV16 L1-derived peptides used in this study and their binding motifs to HLA-A2 and -A24
| |
DR |
A2 |
4A2 |
|||||
|---|---|---|---|---|---|---|---|---|
| position | Amino acid sequence | position | Amino acid sequence | Score | position | Amino acid sequence | Score | |
| Peptide 1 |
54-73 |
KPNNNKILVPKVSGLQYRVF |
60-68 |
ILVPKVSGL |
30 |
59-68 |
KILVPKVSGL |
14 |
| Peptide 2 |
392-422 |
HSMNSTILEDWNFGLQPPPGG |
398-406 |
ILEDWNFGL |
23 |
397-406 |
TILEDWNFGL |
16 |
| Peptide 3 |
62-81 |
VPKVSGLQYRVFRIHLPDPN |
67-75 |
GLQYRVFRI |
22 |
66-75 |
SGLQYRVFRI |
24 |
| Peptide 4 |
112-131 |
PLGVGISGHPLLNKLDDTEN |
118-126 |
SGHPLLNKL |
22 |
117-126 |
ISGHPLLNKL |
12 |
| Peptide 5 |
243-262 |
GDSLFFYLRREQMFVRHLFN |
249-257 |
YLRREQMFV |
22 |
248-257 |
FYLRREQMFV |
12 |
| Peptide 6 |
300-319 |
VTSDAQIFNKPYWLQRAQGH |
305-313 |
QIFNKPYWL |
21 |
305-313 |
QIFNKPYWL |
12 |
| Peptide 7 |
144-162 |
RECISMDYKQTQLCLIGCK |
148-156 |
SMDYKQTQL |
20 |
148-156 |
SMDYKQTQL |
11 |
| Peptide 8 |
293-312 |
PTPSGSMVTSDAQIFNKPYW |
298-306 |
SMVTSDAQI |
20 |
298-306 |
SMVTSDAQI |
10 |
| Peptide 9 |
384-403 |
TADVMTYIHSMNSTILEDWN |
390-399 |
YIHSMNSTIL |
20 |
389-398 |
TYIHSMNSTI |
23 |
| Peptide 10 | 152-171 | KQTQLCLIGCKPPIGEHWG | 157-165 | CLIGCKPPI | 23 | 156-165 | LCLIGCKPPI | 12 |
Anchor residues for HLA class I are shown in boldface.